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KMID : 0043320110340122125
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Archives of Pharmacal Research
2011 Volume.34 No. 12 p.2125 ~ p.2130
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Effects of 4′,7-dimethoxyflavanone on cell cycle arrest and apoptosis in human breast cancer MCF-7 cells
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Choi Eun-Jeong
Lee Jae-In
Kim Gun-Hee
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Abstract
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The present study was designed to investigate the anticancer activity of 4,7-dimethoxyflavanone in vitro. When human breast cancer MCF-7 cells were treated with 4′,7-dimethoxyflavanone at various concentrations (1?200 μM) for 24 h, antiproliferative effects were first observed at 1 μM and the IC50 was 115.62 μM. Conversely, 4′,7-dimethoxyflavanone was not cytotoxic (measured as lactate dehydrogenase release in CHO-K1 cells) under the same conditions. MCF-7 cells exposed to the 4′,7-dimethoxyflavanone at the IC50 concentration showed cell cycle arrest and apoptosis. Compared to the respective control level, exposure to 4′,7-dimethoxyflavanone resulted in a remarkable increase of small DNA fragments at the sub-G1 phase and an increase in the G2/M phase cell population. Moreover, when 4′,7-dimethoxyflavanone treatment caused G2/M phase arrest, an increase in CDK1 together with an increase in cyclin B was observed. Based on these results, 4′,7-dimethoxyflavanone may be a useful anticancer agent.
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KEYWORD
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4′, 7-Dimethoxyflavanone, Anticancer, G2/M phase, CDK1, Cyclin B
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